WebIf you are noticing that the box size of your particles is limiting your reconstruction, (i.e. the FSC is hitting the Nyquist rate), then yes, you can consider using a larger particle size. If … WebJul 15, 2024 · For these results we used a particle box size of 212 Å that was 1.5 times larger than the particle size (~140 Å) and could accommodate delocalized signals from the center of the particle at...
Job: Heterogeneous Refinement - CryoSPARC Guide
WebCenter for Electron Microscopy and Analysis 920 subscribers Subscribe 6.1K views 2 years ago In this episode of Beyond the Scope: CEMAS Discussion Series, CEMAS Cryo-EM Senior Researcher Yoshie... WebHowever, much smaller crystals (~100 nm in size) can be used in MicroED because crystals interact more strongly with electrons than they do with X-rays. This may significantly … st mary\u0027s waverly mn
(PDF) Size matters: optimal mask diameter and box size for single ...
WebMar 4, 2024 · To date, only three macromolecular complexes smaller than 100 kDa have been resolved to high resolution (i.e., better than 4 Å) using cryo-EM SPA: the ~3.8 Å resolution reconstruction of 93 kDa... WebContrast transfer function and defocus gradients in cryo-ET. (A) A simulated CTF curve of a 300-kV microscope with C s = 2.7 mm and a 3-Å pixel size. The left curve is simulated at − 5 μm defocus and the right one at − 500 nm defocus with a phase shift of 90°. WebOct 29, 2024 · A typical CryoEM run may capture a few thousand images, grouped as movies, and may generate a few terabytes of data per sample (median size 1.2 TB). These movies must be stored for further processing. Moreover, the processing steps produce a large amount of intermediate data. st mary\u0027s waverly mn bulletin