WebThe Cre-lox system, derived from P1 bacteriophage, is a potent and specific system for controlling gene expression. The protein Cre recombinase recognizes 34 bp loxP sites, and the orientation and location of the loxP sites determines how the genetic material will be rearranged. The schematic below shows the three types of rearrangements ... Web031332 B6.Cg- Vipr2 em1.1(cre)Hze /J Vipr2-IRES2-Cre-D knock-in mice are designed to have Cre recombinase expression/activity directed by the endogenous vasoactive intestinal peptide receptor 2 promoter/enhancer sequences.
Limitations of the Avp-IRES2-Cre (JAX #023530) and Vip-IRES-Cre …
WebExpression of serotonin 1A and 2A receptors in molecular- and projection-defined neurons of the mouse insular cortex . Anes Ju 1, Beatriz 1Fernandez-Arroyo , Yifan Wu1, Débora Jacky , Anna ... WebPlasmid Slc17a7-IRES2-Cre targeting vector from Dr. Hongkui Zeng's lab contains the insert Slc17a7-IRES2-Cre and is published in Neuron. 2015 Mar 4;85(5):942-58. doi: 10.1016/j.neuron.2015.02.022. This plasmid is available through Addgene. Image: Illustrated plasmid map in PNG format. channel 8 pacific beach news
Limitations of the Avp-IRES2-Cre (JAX #023530) and Vip-IRES-Cre …
WebSlc17a7-IRES2-Cre targeting vector was a gift from Hongkui Zeng (Addgene plasmid # 61574 ; http://n2t.net/addgene:61574 ; RRID:Addgene_61574) For your References section: Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance . Web1) Generation of multigenic-based disease models. The IRES has been successfully used to generate models that mimic various human pathologies. Several cDNAs can be expressed under the control of the same promoter. This results in the co-expression of these genes in the same cells or tissues. WebGeneration of co-expression and/or reporter models. The IRES (internal ribosome entry site) allows the researcher to co-express several genes under the control of the same promoter. Within the past decade, the IRES has been used to develop hundreds of genetically modified models, and is considered to be the "go-to" technology for transgene … channel 8 rochester ny schedule