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Peasy-t5 zero cloning kit

WebOct 1, 2016 · pEASY®-T5 Zero Cloning Kit – CT501. $ 338.00 – $ 673.00 CAD. 0 in stock (expect 7-21 days for delivery) Format. You will earn 16$ if you order this product! $ … WebpEASY® -T5 Zero Cloning Kit pEASY®-T5 Zero Cloning Vector contains a suicide gene. Pricing & AvailabilityDownload CatalogueAdd to wishlist View More Details Add to Cart pEASY® -Uni Seamless Cloning and Assembly Kit No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced.

pEASY-Blunt Simple (linearized) Sequence and Map - SnapGene

WebpEASY cloning kits a proprietary topoisomerase (TOPO) enzyme blend to efficiently clone your DNA in any of the pEASY vectors in only 5 minutes. ... pEASY-Blunt Zero Cloning Kit – CB501 $ 338.00 – $ 898.00 CAD; pEASY-Blunt3 Cloning Kit – CB301 ... pEASY®-T5 Zero Cloning Kit – CT501 $ 338.00 – $ 673.00 CAD; Filter by Supplier. Most ... Webrecovered using a DNA recovery kit (Tiangen Biotech Co., Ltd, Beijing, China). The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. thoma rtl https://euro6carparts.com

Peasy T5 Zero Cloning Kits TransGen biotech co Bioz

WebMay 28, 2024 · The products were ligated to T5 zero cloning vector and transformed with Trans1-T1 into Competent cells by the heat-shock process at 42°C for 30 s (pEASY®-T5 Zero Cloning Kit). Three colonies were randomly picked from each transformation and incubated in Luria-Bertani liquid media at 37°C overnight under continuous shaking. One … WebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … WebJul 11, 2024 · For the sensitivity detection, plasmid pEASY-T5-MDV which contains a 407 bp target segment in the pEASY™-T5 Zero Cloning Vector was constructed and sequenced. The purified positive control pEASY-T5-MDV plasmid (4362 bp) was used as the initial template with a DNA concentration of 31.7 ng/μL. The initial copy number was about 6.7× … thomar story-guest house tomar

CT501-01 pEASY®-T5 Zero Cloning Kit Clinisciences

Category:Cloning Vectors and Kits Civic Bioscience

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Peasy-t5 zero cloning kit

pEASY® -Blunt Zero Cloning Kit - TransGen Biotech

WebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … WebpEASY® -T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain nonrecombinant vector are killed …

Peasy-t5 zero cloning kit

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WebFree Trial pEASY-Blunt Simple (linearized) Cloning vector for blunt-ended PCR products, with blue-white selection, kanamycin and ampicillin resistance markers, and a T7 promoter but … WebIntroduction Product Overview Description of the system The Zero Blunt® PCR Cloning Kit is designed to clone blunt PCR fragments (or any blunt DNA fragment) with a low background of nonrecombinants. The - pCR®-Blunt vector contains the lethal E. coli ccdB gene fused to the C-terminus of LacZα (Bernard et al., 1994).Ligation of a blunt PCR fragment disrupts …

WebpEASY ®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed … WebMar 29, 2024 · Purified PCR product was cloned with pEASY ®-T5 Zero Cloning Kit containing Trans1-T1 Phage-resistant chemically competent cells (Transgen Biotech). Twelve to 80 positive colonies per individual were selected and sequenced bidirectionally with an automatic sequencer (ABI PRISM 3730; Invitrogen Biotechnology Co., Ltd.).

WebpEASY-Blunt Zero (linearized) Cloning vector for blunt-ended PCR products, with a lacZα-ccdB suicide gene that allows for selection of recombinant plasmids. Sequence Author: … WebJan 8, 2016 · The recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Prokaryotic Expression and SDS-PAGE Analysis.

WebEvaluation of pEASY- Uni Seamless Cloning and Assembly Kit to clone multiple fragments of Elaeis guineensis DNA. ... The pEASY™-T5 Zero Cloning Vector (TransGen, Beijing, China) was used for DNA ...

WebThe recovered PCR products were cloned using the pEASY-T5 Zero Cloning Kit (TransGen Biotech (Beijing) Co., Ltd, Beijing, China) by following the product manual. Two positive final clones were submitted to Invitrogen Corporation (Guangzhou, China) for sequencing. Taq 2X PCR Master Mix, DNA Marker, deoxyribonucleoside triphosphates (dNTPs) and ... thoma rwaWeb产品详情介绍. 该载体通过自杀基因表达与否筛选阳性重组子。. 当载体与片段连接成功时，自杀基因无法正确表达，包含重组子的细胞可以正常生长；当载体与片段连接不成功时，自杀基因正确表达，包含载体的细胞无法生长，即“Zero”背景。. 适用于TA克隆 ... thomas0007WebFeb 15, 2024 · 分别以杜梨cDNA和gDNA为模板扩增PbGID1家族基因。扩增程序为：95 ℃预变性2 min；95 ℃ 30 s，55 ℃ 30 s，72 ℃ 1 min，30个循环；72 ℃ 延伸 2 min。PCR产物进行胶回收后，连接至pEASY-Blunt Zero Cloning Kit克隆载体，转化大肠杆菌感受态DH5α中，挑取单克隆测序。 thomas0007 crying